Sopranos actress Edie Falco said last year that it was still possible for the show's creator David Chase to put together a reunion, but little has been heard of the project since.
Van Zandt - who played mob enforcer Silvio Dante on The Sopranos - was asked for an update on a film reunion during an interview on Good Day New York.
"I don't think so. Half the cast is dead," he joked.
The actor-musician went on to remind Good Day New York's hosts that Silvio could return for a possible Sopranos picture since the character was only wounded in the series finale, not killed.
"Just in case the movie does happen, Silvio will be there, but nah, it doesn't look likely, it really doesn't," he commented.
The Sopranos ran for six critically-acclaimed seasons on HBO between 1999 and 2006.
The mob series won 21 Emmy Awards and five Golden Globes.
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Watch Steven Van Zandt discuss The Sopranos below (US only):