The boyband served as musical guests on the April 7 broadcast alongside host and Modern Family actress Sofia Vergara.
Speaking to MTV before their debut performance on the show, group member Harry Styles admitted that the teens were feeling nervous, saying: "I think a lot of it is gonna be a big adrenaline rush. The show's so big over here. I think it's going to be a lot of fun and I think we're gonna have as much fun with it as we can."
Fellow bandmate Niall Horan added: "This is our kind of show. You know, we like to have a bit of a laugh. This is our perfect kind of show [and] we get involved as much as we can."
One Direction recently secured their place in US chart history by becoming the first British group to enter at the top of the Billboard 200 with their debut album, Up All Night.
Watch One Direction sing 'What Makes You Beautiful' on Saturday Night Live below: