Glee star Jane Lynch has already been announced as the roastmaster for the gala, which will be taped on August 4 in Los Angeles.
Stone and Fisher will be joined on the dais by Seth Green, Sons of Anarchy star Katy Sagal and roast favourite Jeffrey Ross.
Comedians Amy Schumer and Anthony Jeselnik will also take part in the gala.
Barr follows in the footsteps of Charlie Sheen, Donald Trump, William Shatner and Pamela Anderson as the honouree of a Comedy Central Roast.
The comic's ex-husband Tom Arnold has reportedly ruled out appearing on the roast given the couple's famously public feud.
The Comedy Central Roast of Roseanne Barr is to air on August 12.
Watch an advertisement for Roseanne Barr's roast below: