The show included two over-enthusiastic signers in a sketch which mocked Mayor Bloomberg's Hurricane Sandy press conferences and his interpreter Lydia Callis, who has become a viral hit.
However, Matlin - who is legally deaf - complained on her Twitter page that the scene was "childish and insulting".
"Millions of deaf people use sign," she said. "Why poke fun/fake it? Poke fun at ME but not the language. Would they do that to Spanish or Chinese? FAIL."
She continued: "I'm referring to sign language parodies. Sign is not mime; it is a legit language.
"As I've said, I don't mind fun poked at me, as I poke back. But at the language, that millions use? It feels childish and insulting."
Matlin also suggested that jokes about sign language could be funny if "done right", saying: "Here's my point. Deaf people HAVE a sense of humour. Ever see me on Family Guy or Seinfeld? But faking signs that we use isn't the same.
"Imagine if a show started making fun of Spanish (using fake Spanish) or Japanese. Do you think they'd actually get away with it? Uh, no..."
Matlin concluded: "The jokes about sign could be funny if done right.. Seinfeld, Family Guy, My Name Is Earl WERE funny. This stuff is just dumb."
Matlin has appeared in shows such as The West Wing and The L Word and came second in The Celebrity Apprentice last year.