However, the actress - who previously appeared in Whedon's Angel and Dollhouse - told TVLine that she is unlikely to land a series regular role on the ABC drama.
"I'm in between the age ranges of the [regular] parts," she explained. "Hopefully I'll get to do something."
Actors confirmed to appear in the S.H.I.E.L.D. pilot include Clark Gregg (The Avengers), Ming-Na Wen (ER), Iain De Caestecker (The Fades) and Elizabeth Henstridge (Hollyoaks).
"I think they're putting together a pretty great cast," said Acker. "I'm excited to watch it."
The 35-year-old also collaborated with Whedon on his 2011 film Cabin in the Woods and stars in the director's forthcoming version of Shakespeare's Much Ado About Nothing.
Summer Glau - who worked with Whedon on Firefly and Dollhouse - has also voiced her desire to appear in the S.H.I.E.L.D. series.
> S.H.I.E.L.D. pilot to shoot in January, says report
> Joss Whedon's S.H.I.E.L.D. TV show - character details revealed
Watch an alternative opening scene for The Avengers below: