House of Cards actor Spacey will lead the guests of the 9th annual post-Academy Awards special, while Tom Hanks, Morgan Freeman and Oscars host Ellen DeGeneres will also make an appearance.
Other famous faces joining this year's show include: Chris and Liam Hemsworth, Christoph Waltz, Ben Kingsley, Mandy Patinkin, Abbie Cornish, Barkhad Abdi, Joseph Gordon-Levitt, Catherine Zeta-Jones, Seth Rogen, Andy Garcia, Adam Driver, Queen Latifah, Gary Oldman and Anjelica Huston.
The special programme will also see the debut of new comedy videos following the success of last year's Movie: The Movie Twovie trailer.
The show will air live on ABC after the 86th Academy Awards and the late local news.
American Hustle and Gravity lead this year's Oscar nominations with ten nods each. Steve McQueen's 12 Years A Slave closely follows with nine.
The ceremony will be held this Sunday, March 2 at the Dolby Theatre in Los Angeles.