US TV
The service will go into beta shortly.
An NBC spokeswoman confirmed promotional content would no longer be available on YouTube. She said it was "not antagonistic" but a move designed to support Hulu.
She said NBC still might put promotional content on YouTube "as we see fit". The network was not "closing the door" on anybody, she added.
YouTube spokesman Ricardo Reyes said: "Our relationship with NBC was a YouTube success story, so we hope NBC decides to post more original content and stay engaged with our users."
Hulu will distribute content on its own standalone site as well as through Yahoo, MSN, AOL, MySpace and other sites.
More: US TV, Video on Demand
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