Bob Dylan's 'Man in the Long Black Coat' and David Bowie's 'Heroes' are among 18 tracks on the LP, up to half of which have been used on the NBC show.
It will feature exclusive new singles including Wilco's 'Glad It's Over', Panic at the Disco's 'Nine in the Afternoon', 'Not Now But Soon' by Imogen Heap, and 'He's Frank' by Brighton Port Authority featuring Iggy Pop.
Wendy & Lisa and the Jesus and Mary Chain will also contribute tracks to the album, which will be available from March 18.
The show's executive producer/director Allan Arkush has created five music video montage podcasts to accompany the new release. The videos, which feature scenes from the show set to the album's songs, will be available to download from the Zune Marketplace online store and for streaming at MSN. The first, Nada Surf's 'Weightless', is available from today.
The NBC Records album will also be released as a CD in selected stores.
Heroes returns for a third series in the US this autumn.