The April 26 episode will see Kabletown refuse to allow Liz Lemon (Tina Fey) to air her sketch comedy series TGS live due to the expensive cost.
Liz and Jack Donaghy (Alec Baldwin) at first appear content to pre-tape TGS, but Kenneth the Page (Jack McBrayer) raises objections and leads the pair on a madcap journey through the history of live television.
Beth McCarthy-Miller will direct the instalment, just as she did with 30 Rock's first live episode in October 2010.
Tracy Morgan, Jane Krakowski and Judah Friedlander will all perform the episode twice on April 26, once for the East Coast and then a second time for the West Coast.
That 2010 live show featured cameos from Matt Damon and Saturday Night Live's Bill Hader. Julia Louis Dreyfus also guest-starred as Liz Lemon in flashback sequences.
30 Rock airs Thursdays at 8.30/7.30c on NBC.
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Watch a clip from the first 30 Rock live episode below: