The network has allegedly maintained that the hit comedy will come to an end if the troubled star is unable to return, according to RadarOnline.
A source told the website: "There has been absolutely no discussion about reworking Two and a Half Men without Charlie. No one is even considering it."
However, these comments contradict reports that emerged earlier this week, suggesting that Warner Bros will instead seek a replacement for Sheen should he not return to the show.
TMZ reported that Sheen's uncle Joe Estevez is planning to meet the show's producer Chuck Lorre and offer himself as a substitute for his nephew until he can make a comeback. Estevez is allegedly keen to have the writers create an episode in which Charlie's character has gone missing - his uncle subsequently arriving to look after his on-screen son.
However, the RadarOnline source maintained that Warner Bros will make no concessions, adding: "At this moment, if Charlie doesn't come back, that's the end of the series."