Jones slammed his CBS sitcom in a taped testimonial for Forerunner Christian Church, calling Two and a Half Men "filth" and urging viewers to stop watching.
"If I am doing any harm, I don't want to be here. I don't want to be contributing to the enemy's plan... You cannot be a true God-fearing person and be on a television show like that," Jones said in the testimonial.
"I know I can't. I'm not okay with what I'm learning, what the Bible says and being on that television show."
His comments have now drawn a response from Supernatural's Padalecki, who chided Jones for accepting a reported $300,000 (£185,000) for each episode of the CBS series.
"AngusTJones -if you BELIEVE what you said, put ur money where ur mouth is and donate ur 'filthy' millions to charity. Try StJude ," Padalecki tweeted on Tuesday (November 27).
© The CW
© CBS
Jones has not issued any public comment on the controversy.
It was previously reported that work on Two and a Half Men is expected to commence as usual today.
