The show stars Emily VanCamp as a young woman who arrives in the Hamptons determined to get revenge on the Grayson clan, the people who destroyed her family.
Nikita star Holmes will play a troublesome Harvard classmate of Daniel Grayson (Josh Bowman), according to Deadline.
The actor previously starred in HBO miniseries The Pacific and has also appeared in episodes of House, Lie to Me and Law & Order: Criminal Intent.
Other actors confirmed to appear in Revenge include James Tupper, Madeleine Stowe, Gabriel Mann, Connor Paolo, Nick Wechsler, Ashley Madekwe, Max Martini and Robbie Amell.
Revenge will premiere on September 21 on ABC.
Watch a trailer for Revenge below: