The theme park includes famous landmarks from Matt Groening's animated Fox TV series and gives visitors the chance to walk the same streets as Homer, Marge, Bart, Lisa and Maggie.
The attraction features Fast Food Boulevard and customers will be able to grab food and drink at Krusty Burger, the Frying Dutchman, Luigi's Pizza, Lard Lad and Moe's Tavern.
A new ride - Kang & Kodos' Twirl 'n' Hurl - takes "foolish humans" on an "intergalactic spin designed to send them into orbit".
"Fans who love both The Simpsons and the ride at Universal Orlando will now be able to experience a much deeper immersion into The Simpsons world with the launch of Springfield," said Jeffrey Godsick, president, Fox Consumer Products.
Simpsons exec producer James L. Brooks added: "I hope everyone has as much pure, irresponsible fun as we did writing the ride, signs and food menus. We raise our Flaming Moe's to ye."