Groff is currently starring in the show as Jesse St James, a love interest for Rachel (Lea Michele), but the character is about to graduate from high school.
However, creator Ryan Murphy told E! Online: "I love his character. We loved [Jonathan]. He and Lea Michele have such great chemistry. I'm sure we'll have him back."
Executive producer Ian Brennan suggested that Jesse could be "one of those freshmen in college who dates a high school girl".
Murphy added: "Maybe he doesn't do so well in college and comes back to get his glory. We're writing the script right now!"
Glee continues tonight at 9pm on Fox in the US and Mondays at 9pm on E4 in the UK.