According to Entertainment Weekly's Michael Ausiello, the former Full House star is "nearing a deal" to appear in the next season of the Fox comedy.
It is thought that he will play a dentist and possible new love interest for Emma (Jayma Mays).
Stamos shot to fame as Uncle Jesse in Full House - which aired from 1987 to 1995 - and went on to star as Dr Tony Gates in ER between 2005 and 2009. He recently appeared in a Broadway revival of Bye Bye Birdie.
The season finale of Glee airs tonight at 9pm on Fox in the US and on Monday at 9pm on E4 in the UK.