Last summer, Kings of Leon star Jared Followill revealed that the band had turned down the chance for their songs to appear on Ugly Betty and Glee because they were concerned about 'selling out'.
In an interview with The Hollywood Reporter, Murphy admitted that the remarks annoyed him.
"F**k you, Kings of Leon," he said. "They're self-centred a**holes, and they missed the big picture. They missed that a 7-year-old kid can see someone close to their age singing a Kings of Leon song, which will maybe make them want to join a glee club or pick up a musical instrument. It's like, OK, hate on arts education. You can make fun of Glee all you want, but at its heart, what we really do is turn kids on to music."
Murphy was also irritated by Slash's recent claim that "Glee is worse than Grease", saying: "Usually I find that people who make those comments, their careers are over - they're uneducated and stupid."
However, Kings of Leon star Caleb Followill admitted that he is surprised the band's refusal to let the show use their songs has annoyed Murphy so much.
"This whole Glee thing is a shock to us," he said. "It's gotten out of hand. At the time of the request, we hadn't even seen the show. It came at the end of that record cycle and we were over promoting ['Use Somebody']. This was never meant as a slap in the face to Glee or to music education or to fans of the show. We're not sure where the anger is coming from."
Glee returns for a new episode in the US on February 6 after the Super Bowl on Fox. The show continues in the UK on Mondays at 9pm on E4.