The two Glee actresses, who play Brittany and Santana respectively on the Fox series, shared a kiss as part of the final show in the hugely successful tour.
In a skit for the show, Morris was left alone on stage after accidentally mistaking Darren Criss's Blaine for being straight. Rivera then made her appearance to give Morris a tender kiss.
In addition, following Morris and Rivera's unplanned kiss, Criss decided to shock Chris Colfer's Kurt with a kiss as well.
Gwyneth Paltrow previously surprised 'Glee Live!' audiences by reprising her popular guest role as substitute teacher Holly Holliday on stage to sing her cover of 'Forget You'.
Glee begins filming its third season in August.
Watch the kisses between Heather Morris and Naya Rivera, as well as Darren Criss and Chris Colfer, below: