The White Collar star is nearing a deal to play the brother of Blaine (Darren Criss), according to TVLine.
The character will debut in an April episode and will reportedly share a musical number with his brother.
Bomer has played Neal Caffrey on USA's White Collar since 2009 and previously played the recurring role of Bryce Larkin on NBC's Chuck.
He will also collaborate with Glee creator Ryan Murphy on forthcoming film drama The Normal Heart.
Jeff Goldblum and Brian Stokes Mitchell recently joined the Glee cast. The pair will play Rachel (Lea Michele)'s gay dads.
Glee airs on Tuesdays at 8/7c on Fox.
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Watch a preview for Glee's forthcoming Michael Jackson special below: