The Melrose Place star will first appear in the show in episode 18, reports TV Line.
Puck's (Mark Salling) mum, played by Gina Hecht, appeared in the first season episode 'Mash-Up'.
Calabro is the latest star to sign up for a guest stint in the show, which has lined up a role for White Collar's Matt Bomer as Blaine's (Darren Criss) brother next month.
Other guest stars who have appeared or still to appear in season three include Jeff Goldblum, Brian Stokes Mitchell, Ricky Martin and Gloria Estefan.
Glee returns to Fox on April 10 with new episode 'Big Brother'.
The show is currently airing in the UK on Thursday nights on Sky1.
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