Murphy confirmed on Twitter that he had invited the Grammy-winning star to appear on his Fox musical drama at Monday night's (October 15) God's Love We Deliver Golden Heart Awards.
"I just asked the incredible Bette Midler to be on Glee in front of 1,000 people," he wrote. "I hope she says yes!"
The showrunner later added: "Bette said yes! Amazing. I am plotting... who should she play?"
Murphy also confirmed news that American Idol's Jessica Sanchez had been cast on Glee and teased another possible themed episode of the show.
"With voices like Jessica, Lea [Michele], Naya [Rivera], Melissa [Benoist], Amber [Riley], and Jenna [Ushkowitz]... [Barbra] Streisand episode?" he posted.
Glee is currently on a month's hiatus and will return to Fox on November 8. The series airs on Sky1 in the UK.
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Watch a promo for season four of Glee below: