The Blade Runner star will play the role of Macklyn on the HBO drama's sixth season, TVLine reports.
The sinister figure is said to have a mysterious connection to siblings Sookie (Anna Paquin) and Jason Stackhouse (Ryan Kwanten).
68-year-old Hauer is best known for his film credits, including roles in 1982's Blade Runner, 1986's The Hitcher and 2005's Batman Begins.
His past television appearances include guest stints on Smallville and Alias and a Golden Globe-winning performance in 1987 TV movie Escape From Sobibor.
It was previously confirmed that the sixth season of True Blood will be comprised of just 10 episodes - two less than in previous years - due in part to star Anna Paquin recently giving birth to twins.
The series is expected to return to HBO in June 2013 and airs on FX in the UK.