It was previously reported that the sixth episode of the new season will poke fun at the vampire franchise.
Spoiler TV reports that the instalment will feature a vampire named Robert, intended as a parody of Twilight star Robert Pattinson.
A character named Boris, described as a "large and frightening" vampire, will also feature.
Jensen Ackles - who plays Dean Winchester on the show - previously joked that Twilight fans are "crazy" and "have [no] self-respect".
Supernatural returns to The CW on September 24.