The eighth episode of the CW drama's seventh season bears the unusual title, 'Season Seven, Time for a Wedding!'.
"The gist of it is that these characters have been through a lot," Padalecki told Entertainment Weekly. "Dean (Jensen Ackles) in relationships and [my character] Sam in relationships, and now - it kind of comes out of the blue - but there is going to be a character who gets married."
Padalecki hinted that it will indeed be either Dean or Sam walking down the aisle.
"It's kind of confusing to the other character, naturally," he explained. "But it all spells itself out pretty quickly."
DJ Qualls and Kevin McNally recently signed up for roles in Supernatural's seventh season, while former Buffy stars James Marsters and Charisma Carpenter will appear in the fifth episode as a husband and wife.
Supernatural continues on Fridays at 9/8c on The CW.
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Watch a preview for the next episode of Supernatural below: