Rebekah was daggered by Elena (Nina Dobrev) earlier this season but Elijah (Daniel Gillies) resurrected her in this week's instalment.
"Elena stuck a dagger in Rebekah's back so she's not going to be happy about that," Holt told TV Guide. "I think she's going to come and stir the pot a little bit."
Holt added that Klaus (Joseph Morgan) will "feel some aggression aimed towards him" from Rebekah, adding: "I think she's so unhappy about the fact that he lied to her all this time and she feels betrayed by him and I think he's really going to feel that from her."
Holt also suggested that the reunion of the Original family members is "going to be fun" and said that Rebekah is "really excited" that her mother has been resurrected.
"She feels like a family unit and sort of a powerful unit," she said. "So I think it's really going to mix it up a bit in Mystic Falls."
Morgan recently admitted that he is hoping for a confrontation between Klaus and Rebekah in an upcoming episode.
The Vampire Diaries airs on Thursdays at 8/7c on The CW.