The ABC reality series will debut with an all-star lineup of contestants from past seasons of the show.
Zap2It reports via a press release from ABC that Bieber will hit the stage for a performance of his new single 'As Long As You Love Me' from his album Believe during the two-hour premiere.
Bieber recently unveiled the song during a live performance on another reality competition show, America's Got Talent.
Rap star Pitbull will also make an appearance during the show, debuting his new single 'Don't Stop the Party'.
Dancing With the Stars: All-Stars debuts on September 24, 8pm ET on ABC.
Watch the video for Justin Bieber's 'As Long As You Love Me' below: